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For semi-quantitative RT-PCR analyses, a limited number of cycles were conducted with a 1:5 diluted cDNA template, removing samples at the indicated cycle (72C step) and transfer to another thermocycler for completing the final elongation step at 72C for 5 min (and then kept on ice)

Exclusion criteria were as follows: medical conditions precluding physical testing and/or affecting the ability to complete questionnaires, no previous diagnose of sleep disorders and no history of cancer in the last 5 years

The FDA maintains import alerts for glutathione injections marketed for skin whitening, particularly from manufacturers in Asia and the Middle East

Jin SG, Wu X, Li AX, et al